Identification of <i>UFGT</i>-gene-associated groups of <i>Vitis vinifera</i> L. by the developed method of PCR-RFLP genotyping of grape

The Vitis vinifera L. UFGT gene is one of the diagnostically significant genes for genetic testing technical grape varieties as well wine materials and wines produced from them. strategy identification DNA authentication that was previously developed by us based on direct sequencing specific PCR product with a length 99 bp gave an impulse to prognostic assessment feasibility PCR-RFLP analysis detection five polymorphic positions following 13 gene-associated groups aim this work consisted in using method genotyping grape. Objects research were 24 samples varieties. Their sample preparation carried out extracting 50–100 mg mature pulp or stone its mechanical comminution mortar transfer Eppendorf-type tube. Then, nucleic acids extracted commercial innuPREP Plant Kit DiamondDNA kit. performed Phire Direct Master Mix four selected restrictases ( PstI , BsaXI BtsIMutI HinfI ) according protocols presented paper. fragments visualization electropherograms UV transilluminator after horizontal electrophoresis 2.5% agarose gel stained TAE buffer. grapes specially detecting demonstrated when With that, positive result achieved due practical ability each discriminate strictly specified position generating characteristic profiles L., seven which revealed during study. Therefore, study, genotypic affiliation several tested established: six identified representatives group No.1; assigned No.2; two characterized trait associated No.3; belonged No. 4; 5; No.13.